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PEŇÁZOVÁ, E. -- DVOŘÁK, M. -- RAGASOVÁ, L. -- KISS, T. -- PEČENKA, J. -- ČECHOVÁ, J. -- EICHMEIER, A. Multiplex real-time PCR for the detection of Clavibacter michiganensis subsp. michiganensis, Pseudomonas syringae pv. tomato and pathogenic Xanthomonas species on tomato plants. PLoS One. 2020. v. 15, no. 1, ISSN 1932-6203. URL: https://doi.org/10.1371/journal.pone.0227559

Original name: Multiplex real-time PCR for the detection of Clavibacter michiganensis subsp. michiganensis, Pseudomonas syringae pv. tomato and pathogenic Xanthomonas species on tomato plants
Czech name:
Written by (author):
Department:
Mendeleum - Institute of Genetics and Plant Breeding
Department of Vegetable Growing and Floriculture
Department of Forest Protection and Wildlife Management
Kind of publication: article in a professional periodical
Periodical:
PLoS One
Nature of article: paper
Volume no. (year):
15
Periodical number within the volume:
1
Year of publication:
2020
Starting page:
Up to page:
Number of pages:
15
Sub-specification:
článek je obsažen v databázi Web of Science
UT code by Web of Science:
EID code by Scopus:
Form of publication:
printed version
Original language: English
Description in original language: A multiplex real-time PCR method based on fluorescent TaqMan(R) probes was developed for the simultaneous detection of the tomato pathogenic bacteria Clavibacter michiganensis subsp. michiganensis, Pseudomonas syringae pv. tomato and bacterial spot-causing xanthomonads. The specificity of the multiplex assay was validated on 44 bacterial strains, including 32 target pathogen strains as well as closely related species and nontarget tomato pathogenic bacteria. The designed multiplex real-time PCR showed high sensitivity when positive amplification was observed for one pg of bacterial DNA in the cases of Clavibacter michiganensis subsp. michiganensis and Pseudomonas syringae pv. tomato bacteria and 100 pg for bacterial spot-causing xanthomonads. The reliability of the developed multiplex real-time PCR assay for in planta detection was verified by recognition of the target pathogens in 18 tomato plants artificially inoculated by each of the target bacteria and tomato samples from production greenhouses.
Description in English:
Description in Czech:
Year of submission:
2020
Year of transmission:
RIV identification number:
URL:
https://doi.org/10.1371/journal.pone.0227559
 
Entry made by:
Last change:
02/07/2020 10:16 (Markéta Hejčová, DiS.)

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Source specification:

PLoS One. ISSN 1932-6203.

Original name:
PLoS One
English name:
Czech name:
Written by (author):
Kind of publication: magazine
ISSN:
1932-6203
Country of publisher: United States of America
Place of publishing:
Publisher:
Public Library of Science
URL:
Reviewed magazine:
no
Original language:
Description in original language:
Description in English:
Description in Czech:
 
Entry made by:
Last change: 03/14/2012 20:32 (Ing. Aleš Kutín)

Evaluation of publication:

1
2
345
        
1
2
345
bad
 
good
        
uninteresting
 
interesting
Assessed: 0
Average score: -
        Assessed: 0Average score: -
1
2345
        
1
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45
amateur
 
professional        theoretical
 
practical
Assessed: 0
Average score: -
        
Assessed: 0
Average score: -