Mendel University in Brno - list of publications


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PEČENKA, J. -- KOCANOVÁ, M. -- BARÁNEK, M. -- GAZDÍK, F. -- RAGASOVÁ, L. -- PEŇÁZOVÁ, E. -- ČECHOVÁ, J. -- BERAN, P. -- EICHMEIER, A. Species-specific PCR primers for the detection of poorly distinguishable Xanthomonas euvesicatoria. Crop protection. 2020. v. 127, no. January, ISSN 0261-2194. URL: https://doi.org/10.1016/j.cropro.2019.104978

Original name: Species-specific PCR primers for the detection of poorly distinguishable Xanthomonas euvesicatoria
Czech name:
Written by (author): Ing. Jakub Pečenka
Ing. Mária Kocanová
doc. Mgr. Miroslav Baránek, Ph.D.
Ing. Filip Gazdík
Ing. Lucia Ragasová
Ing. Bc. Eliška Peňázová, Ph.D.
Ing. Jana Čechová, Ph.D.
Pavel Beran
Ing. Aleš Eichmeier, Ph.D.
Department: Mendeleum - Institute of Genetics and Plant Breeding
Kind of publication: article in a professional periodical
Periodical: Crop protection
Nature of article: paper
Volume no. (year): 127
Periodical number within the volume: January
Year of publication: 2020
Starting page:
Up to page:
Number of pages: 5
Sub-specification: článek je obsažen v databázi Web of Science
UT code by Web of Science:
EID code by Scopus:
Form of publication: printed version
Original language: English
Description in original language: New species-specific PCR primers for detection of bacterium Xanthomonas euvesicatoria, the causal agent of bacterial spot of tomato and pepper, were designed in this work. Developed PCR primer pair ZnDoF/ZnDoR partially targets the coding sequence of Zn-dependent oxidoreductase gene. The primer pair was tested on various bacterial strains including bacteria from the bacterial spot-causing xanthomonads (BSX) group. Designed primers showed to be species-specific for the samples containing DNA of X. euvesicatoria. Specificity of the designed primers was also compared with the primer pairs already published. Subsequently, sensitivity of ZnDoF/ZnDoR primers was determined by ten-fold dilution of standardized sample. Detection capability of developed primers was also investigated on artificially infected pepper plants and seeds collected from bacterial spot-like peppers. Primers proved to be species-specific for in planta and in seed detection as well as to distinguish X. euvesicatoria from other bacterial strains used in this assay.
Description in English:
Description in Czech:
Year of submission: 2020
Year of transmission:
RIV identification number:
URL: https://doi.org/10.1016/j.cropro.2019.104978
 
Entry made by: Markéta Hejčová, DiS.
Last change: 10/16/2019 10:15 (Markéta Hejčová, DiS.)

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Source specification:

Crop protection. Oxford: ISSN 0261-2194.

Original name: Crop protection
Czech name:
Written by (author):
Kind of publication: magazine
ISSN: 0261-2194
Country of publisher: United Kingdom of Great Britain and Northern Ireland
Place of publishing: Oxford
Publisher: Elsevier
URL: http://www.journals.elsevier.com/crop-protection
Reviewed magazine: no
Original language: English
Description in original language:
Description in English:
Description in Czech:
 
Entry made by: Ing. Jitka Dejmalová, Ph.D.
Last change: 10/05/2017 10:54 (Šárka Novotná)

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