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ANANBEH, H A A A. -- MERLOS RODRIGO, M Á. -- JELÍNKOVÁ, P. -- STRMISKA, V. -- ŠPLÍCHAL, Z. -- JEHMLICH, N. -- MICHÁLKOVÁ, H. -- STOJANOVIĆ, M. -- VOBĚRKOVÁ, S. -- ADAM, V. -- MOULICK, A. Soil protein as a potential antimicrobial agent against methicillin -resistant Staphylococcus aureus. Environmental Research. 2020. v. 188, no. September, ISSN 0013-9351. URL: https://doi.org/10.1016/j.envres.2020.109320

Original name: Soil protein as a potential antimicrobial agent against methicillin -resistant Staphylococcus aureus
Czech name:
Written by (author):
Department:
Department of Chemistry and Biochemistry
CEITEC MENDELU
Kind of publication: article in a professional periodical
Periodical:
Environmental Research
Nature of article: paper
Volume no. (year):
188
Periodical number within the volume:
September
Year of publication:
2020
Starting page:
Up to page:
Number of pages:
13
Sub-specification:
článek je obsažen v databázi Web of Science
UT code by Web of Science:
EID code by Scopus:
Form of publication:
printed version
Original language:
English
Description in original language:
Recently, the interest is increasing to find alternatives to replace the usage of antibiotics since their massive and improper usage enhance the antibiotic resistance in human pathogens. In this study, for the first time we showed that the soil proteins have very high antibacterial activity (98% of growth inhibition) against methicillin resistant Staphylococcus aureus (MRSA), one of the most threatening human pathogens. We found that the protein extract (C3) from the forest with past intensive management showed higher antibacterial activity than that of unmanaged forest. The MIC and IC50 were found to be 30 and 15.0 μg protein gMINUS SIGN 1 dry soil respectively. C3 was found to kill the bacteria by cell wall disruption and genotoxicity which was confirmed by optical and fluorescent microscopy and comet assay. According to qPCR study, the mecA (the antibiotic resistant gene) expression in MRSA was found to be down-regulated after C3 treatment. In contrast, C3 showed no hemolytic toxicity on human red blood cells which was confirmed by hemolytic assay. According to ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS), 144 proteins were identified in C3 among which the majority belonged to Gram negative bacteria (45.8%). Altogether, our results will help to develop novel, cost-effective, non-toxic and highly efficient antibacterial medicines from natural sources against antibiotic resistant infections.
Description in English:
Description in Czech:
Year of submission:
2020
Year of transmission:
RIV identification number:
URL:
https://doi.org/10.1016/j.envres.2020.109320
 
Entry made by:
Last change:
07/01/2020 16:53 (Markéta Hejčová, DiS.)

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Source specification:

Environmental Research. San Diego: ISSN 0013-9351.

Original name:
Environmental Research
Czech name:
Written by (author):
Kind of publication:
magazine
ISSN:
0013-9351
Country of publisher:
United States of America
Place of publishing:
San Diego
Publisher:
Academic Press inc. Elsevier Science,
URL:
https://www.journals.elsevier.com/environmental-research
Reviewed magazine:
no
Original language: English
Description in original language:
Description in English:
Description in Czech:
 
Entry made by: Hanadi Abd Alrahman Ali Ananbeh, Ph.D.
Last change:
03/04/2020 11:36 (Šárka Novotná)

Evaluation of publication:

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