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KUDR, J. -- ZHAO, L. -- NGUYEN, E P. -- AROLA, H. -- NEVANEN, T K. -- ADAM, V. -- ZÍTKA, O. -- MERKOÇI, A. Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing. Biosensors and Bioelectronics. 2020. v. 156, no. 15 May, ISSN 0956-5663. URL: https://doi.org/10.1016/j.bios.2020.112109

Original name:
Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing
Czech name:
Written by (author):
Ing. Jiří Kudr, Ph.D.
Lei Zhao
Emily P Nguyen
Henri Arola
Tarja K Nevanen
prof. RNDr. Vojtěch Adam, Ph.D.
doc. RNDr. Ondřej Zítka, Ph.D.
Arben Merkoçi
Department:
Department of Chemistry and Biochemistry
Kind of publication:
article in a professional periodical
Periodical:
Biosensors and Bioelectronics
Nature of article:
paper
Volume no. (year): 156
Periodical number within the volume:
15 May
Year of publication:
2020
Starting page:
Up to page:
Number of pages:
Sub-specification:
článek je obsažen v databázi Web of Science
UT code by Web of Science:
EID code by Scopus:
Form of publication:
printed version
Original language:
English
Description in original language:
The design and application of an inkjet-printed electrochemically reduced graphene oxide microelectrode for HT-2 mycotoxin immunoenzymatic biosensing is reported. A water-based graphene oxide ink was first formulated and single-drop line working microelectrodes were inkjet-printed onto poly(ethylene 2,6-naphthalate) substrates, with dimensions of 78 μm in width and 30 nm in height after solvent evaporation. The printed graphene oxide microelectrodes were electrochemically reduced and characterized by Raman and X-ray photoelectron spectroscopies in addition to microscopies. Through optimization of the electrochemical reduction parameters, differential pulse voltammetry were performed to examine the sensing of 1-naphthol (1-N), where it was revealed that reduction times had significant effects on electrode performance. The developed microelectrodes were then used as an immunoenzymatic biosensor for the detection of HT-2 mycotoxin based on carbodiimide linking of the microelectrode surface and HT-2 toxin antigen binding fragment of antibody (anti-HT2 (10) Fab). The HT-2 toxin and anti-HT2 (10) Fab reaction was reported by anti-HT2 immune complex single-chain variable fragment of antibody fused with alkaline phosphatase (anti-IC-HT2 scFv-ALP) which is able to produce an electroactive reporter - 1-N. The biosensor showed detection limit of 1.6 ng BULLET OPERATOR mLMINUS SIGN 1 and a linear dynamic range of 6.3 - 100.0 ng BULLET OPERATOR mLMINUS SIGN 1 within a 5 min incubation with 1-naphthyl phosphate (1-NP) substrate.
Description in English:
Description in Czech:
Year of submission: 2020
Year of transmission:
RIV identification number:
URL:
https://doi.org/10.1016/j.bios.2020.112109
 
Entry made by: Markéta Hejčová, DiS.
Last change:
03/30/2020 15:51 (Markéta Hejčová, DiS.)

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Source specification:

Biosensors and Bioelectronics. ISSN 0956-5663.

Original name:
Biosensors and Bioelectronics
English name:
Czech name:
Written by (author):
Kind of publication: magazine
ISSN: 0956-5663
Country of publisher: Kingdom of the Netherlands
Place of publishing:
Publisher: Elsevier ADVANCED TECHNOLOGY
URL:
Reviewed magazine: no
Original language:
Description in original language:
Description in English:
Description in Czech:
 
Entry made by:
Last change:
03/14/2012 20:39 (Ing. Aleš Kutín)

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