Přehled vypsaných témat - Ústav ochrany lesů a myslivosti (LDF)Help

Základní údaje

Typ práce:
Bakalářská práce
Název tématu: Detection of RNA viruses by RT-PCR in forest fungal pathogens
Stav tématu:
schváleno (prof. Dr. Ing. Libor Jankovský - vedoucí pracoviště)
Vedoucí práce:
Lesnická a dřevařská fakulta
Garantující pracoviště: Ústav ochrany lesů a myslivosti (LDF)
Max. počet studentů:
Dr. Leticia Botella Sánchez
In the global current scenario, where the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak is a serious health concern, several scientific terms have become very popular and commonly used: RNA, DNA, cDNA, PCR, qPCR, RT-PCR, RT-qPCR, ELISA, sample, outbreak, virus transmission, virus infection, asymptomatic etc. All these terms are also applied and used in forest pathology, in particular by those who work on viruses infecting fungal forest pathogens. Did you know that also fungal forest pathogens host RNA viruses? Viruses (mycoviruses) are widespread in all major groups of fungi and oomycetes. They are primarily double-stranded (ds) or positive-sense single-stranded (ss) RNA. But, lately negative ssRNA, few ssDNA, dsDNA viruses and virus-like RNA reverse transcribing genomes (retrotransposons) have been also described. The majority of mycoviruses are considered ‘cryptic’ (latent) because they do not seem to produce phenotypic alterations in their fungal hosts. However, some mycoviruses decrease fungal virulence, pigmentation, and sporulation. The most important example is Cryphonectria hypovirus 1 (CHV1), a (+) ssRNA virus, which has decisively restricted chestnut blight in many areas in Europe through its naturally or artificially controlled spread. Likewise, other antagonistic mycoviruses have been described within the fungal genera Alternaria, Botrytis, Diaporthe, Fusarium, Helicobasidium, Heterobasidion, Ophiostoma, Rhizoctonia, Rosellinia and Sclerotinia. Here, I would like to offer you the possibility of learning how known RNA viruses are detected by RT-PCR in several forest pathogens, such as Dothistroma spp., Heterobasidion annosum, Gremmeniella abietina and/or Phytophthora castaneae. You will also learn how to do other types or RNA extractions that are useful for the detection of viruses, while you get fully introduced in molecular biology. Don’t be scare of English! I can also speak a bit of Czech!!!!

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