|Proline effect on Gypsy moth (Lymantria dispar L.) (Lepidoptera: Lymantriidae) larval performance|
schváleno (prof. Dr. Ing. Libor Jankovský - vedoucí pracoviště)
|Slobodan Milanović, Ph.D.|
|Lesnická a dřevařská fakulta|
Ústav ochrany lesů a myslivosti (LDF)
|Max. počet studentů:||2|
Outbreaks of herbivorous insects can, theoretically, be caused by stresses to host plants that cause increases in the concentration of certain amino acids crucial to the fecundity or survival of insects (White 1978). Increases in free amino acids consistently follow stress, but the total nitrogen concentration of plant tissue may not vary with stress. Insects absorb nitrogen through the gut primarily in the form of free amino acids or small peptides. Thus, the initial cost of proteolysis is saved if amino acids are ingested in this form. Much of the increase is likely to involve nonessential amino acids: proline, glutamine, or asparagine. Proline has osmoregulatory or dehydration resistance functions at cellular level in the cytoplasm (Le Rudlier et al., 1984). Free amino acid levels also increase following salinity exposure in some plants (Jeffries at al., 1979), ectoparasitic infection (Epstein and Cohn, 1971), nutrient depletion (Court at al., 1972), and/or exposure to pollutants (Malhotra and Sarkar, 1979). The nutritional value to insects of nonessential amino acids that are increased by stress is unclear; there may be indirect advantages, but these have not been well established. Effect of different concentration of Proline on the GM performance would be tested by adding proline into artificial diet during fourth larval instar. In performance test, duration of fourth larval instar, relative consumption rate (RCR), relative growth rate (RGR), assimilation efficacy (AD), efficacy of conversion of digested food(ECD) and efficacy of conversion of ingested food (ECI) into a larval body mass will be measured according to the Waldbauer (1968.
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