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doc. Mgr. Miroslav Baránek, Ph.D.
Identification number: 3560
University e-mail: miroslav.baranek [at] mendelu.cz
 
Academic staff - Associate Professor - Mendeleum - Institute of Genetics and Plant Breeding (FH)
Head of department - Mendeleum - Institute of Genetics and Plant Breeding (FH)

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The following list contains all information available to the publication.

PEČENKA, J. -- KOCANOVÁ, M. -- BARÁNEK, M. -- GAZDÍK, F. -- RAGASOVÁ, L. -- PEŇÁZOVÁ, E. -- ČECHOVÁ, J. -- BERAN, P. -- EICHMEIER, A. Species-specific PCR primers for the detection of poorly distinguishable Xanthomonas euvesicatoria. Crop protection. 2020. v. 127, no. January, ISSN 0261-2194. URL: https://doi.org/10.1016/j.cropro.2019.104978

Original name: Species-specific PCR primers for the detection of poorly distinguishable Xanthomonas euvesicatoria
Czech name:
Written by (author):
Department:
Mendeleum - Institute of Genetics and Plant Breeding
Kind of publication:
article in a professional periodical
Periodical:
Crop protection
Nature of article:
paper
Volume no. (year):
127
Periodical number within the volume:
January
Year of publication:
2020
Starting page:
Up to page:
Number of pages: 5
Sub-specification:
článek je obsažen v databázi Web of Science
UT code by Web of Science:
EID code by Scopus:
Form of publication:
printed version
Original language:
English
Description in original language:
New species-specific PCR primers for detection of bacterium Xanthomonas euvesicatoria, the causal agent of bacterial spot of tomato and pepper, were designed in this work. Developed PCR primer pair ZnDoF/ZnDoR partially targets the coding sequence of Zn-dependent oxidoreductase gene. The primer pair was tested on various bacterial strains including bacteria from the bacterial spot-causing xanthomonads (BSX) group. Designed primers showed to be species-specific for the samples containing DNA of X. euvesicatoria. Specificity of the designed primers was also compared with the primer pairs already published. Subsequently, sensitivity of ZnDoF/ZnDoR primers was determined by ten-fold dilution of standardized sample. Detection capability of developed primers was also investigated on artificially infected pepper plants and seeds collected from bacterial spot-like peppers. Primers proved to be species-specific for in planta and in seed detection as well as to distinguish X. euvesicatoria from other bacterial strains used in this assay.
Description in English:
Description in Czech:
Year of submission:
2020
Year of transmission:
RIV identification number:
URL:
https://doi.org/10.1016/j.cropro.2019.104978
 
Entry made by: Markéta Hejčová, DiS.
Last change: 10/16/2019 10:15 (Markéta Hejčová, DiS.)

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Source specification:

Crop protection. Oxford: ISSN 0261-2194.

Original name:
Crop protection
Czech name:
Written by (author):
Kind of publication: magazine
ISSN:
0261-2194
Country of publisher:
United Kingdom of Great Britain and Northern Ireland
Place of publishing:
Oxford
Publisher:
Elsevier
URL:
http://www.journals.elsevier.com/crop-protection
Reviewed magazine:
no
Original language:
English
Description in original language:
Description in English:
Description in Czech:
 
Entry made by:
Last change:
10/05/2017 10:54 (Šárka Novotná)

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