Course syllabus MTRP - Microscopic Techniques of Plant Tissue (FFWT - WS 2019/2020)

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Course code: MTRP
Course title in language of instruction: Mikroskopické techniky rostlinných pletiv
Course title in Czech: Microscopic Techniques of Plant Tissue
Course title in English: Microscopic Techniques of Plant Tissue
Mode of completion and number of credits: Fulfillment of requirements (3 credits)
(1 ECTS credit = 28 hours of workload)
Mode of delivery/Timetabled classes: full-time, 0/2 (hours of lectures per week / hours of seminars per week)
part-time, 16/0 (lectures per period / seminars per period)
Language of instruction: Czech
Level of course: bachelor; master continuing
Semester: WS 2019/2020
Name of lecturer: doc. Ing. Vladimír Gryc, Ph.D. (examiner, instructor, lecturer, supervisor)
doc. Ing. Hanuš Vavrčík, Ph.D. (examiner, instructor, lecturer)
Prerequisites: not State Bachelor Examination
Aims of the course:
The goal is to provide information about microscopic techniques using in making of permanent microslides of plant tissues. The subject is especially focused on secondary tissues (xylem and phloem) and secondary meristematic tissue -- cambium. Student will be able to make and analyse microslides after course alone.
Course contents:
1.Introduction to subject, safety in laboratory (allowance 0/2)
b.Safety in laboratory
c.Chemical substances law

2.Microscopic structure of woody species stem (allowance 0/2)
a.The structure of softwoods
b.The structure of hardwoods

3.Wood identification (allowance 0/2)
a.Macroscopic wood identification
b.Microscopic wood identification
c.Wood lexicon

4.Methods of microslides making from plant tissue (allowance 0/2)
b.Preparation of samples before sectioning
c.Sectioning on microtome
d.Staining of microsections
e.Making of temporal and permanent microslides

5.Practical making of temporal microslides (allowance 0/2)
6.Practical making of permanent microslides of softwoods (allowance 0/2)
7.Practical making of permanent microslides of hardwoods (allowance 0/4)
8.Making of permanent microslides of phloem, xylem and cambial zone of woody species (allowance 0/4)
9.Practical making of permanent microslides from cores (allowance 0/2)
10.Presentation of microphotographs (allowance 0/2)
11.Digitalization of microslides and image analysis (allowance 0/4)
a.Digitalization of microslides
b.Image analysis (WinCELL, ImageJ software)

Learning outcomes and competences:
Generic competences:
-Ability to analyse and synthesize
-Ability to create new ideas (creativity)
-Ability to work independently
-Capacity to adapt to new situations
-Capacity to learn
-Ethical commitment
-General knowledge
-Interpersonal skills
-Professional knowledge
-Quality concept awareness
-Science and research skills
-Skilled at utilizing and processing information

Specific competences:
-Ability to make pernament microslides
-Ability to use image analysis software
-Ability work with microscope
-Ability work with microtome
-Analysis of microscopic section

Type of course unit: optional
Year of study: Not applicable - the subject could be chosen at anytime during the course of the programme.
Work placement: There is no compulsory work placement in the course unit.
Recommended study modules: -
Learning activities and study load (hours of study load):
Type of teaching methodDaily attendanceCombined form
Direct teaching
     practice28 h0 h
     consultation4 h14 h
     preparation for regular assessment10 h22 h
     preparation of presentation5 h11 h
     elaboration and execution of projects25 h25 h
     writing of seminar paper12 h12 h
Total84 h84 h
Assessment methods:
semestral project -- making of permanent and temporal microslides and documentary snapshots, analyzing of sections.
Recommended reading:
TypeAuthorTitlePublished inPublisherYearISBN
RQŠLEZINGEROVÁ, J. -- GANDELOVÁ, L.Stavba dřevaBrnoMendelova zemědělská a lesnická univerzita v Brně200280-7157-636-0
RQŠLEZINGEROVÁ, J. -- GANDELOVÁ, L.Stavba dřeva (cvičení)BrnoMZLU v Brně2008978-80-7375-168-5
RQVAVRČÍK, H. -- GRYC, V.Metodika výroby mikroskopických preparátů ze vzorků dřeva2004ISSN 1211-8516
REWAGENFÜHR, R.Anatomie des Holzes : Strukturanalytik - Identifizierung - Nomenklatur - MikrotechnologieLeinfelden-EchterdingenDRW Verlag19993-87181-351-6
REVAVRČÍK, H.Analýza růstu xylému na buněčné úrovni s využitím fluorescenční mikroskopie2002
RESCHWEINGRUBER, F H.Anatomie europäischer Hölzer/Anatomy of European woods : Ein Atlas zur Bestimmung europäischer Baum-, Strauch- und ZwergstrauchhölzerBernVerlag Paul Haupt19903-258-04258-6
RESCHWEINGRUBER, F H.Wood structure and environmentBerlinSpringer978-3-540-48299-4
RESCHWEINGRUBER, F H. -- SCHULZE, E.Atlas of woody plant stems: evolution, structure, and environmental modificationsBerlinSpringer20063-540-32523-9


Last modification made by Ing. Alice Malá on 05/20/2019.

Type of output: