Course syllabus MGEZI - Animal Molecular Genetics (FA - WS 2017/2018)

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Course code: MGEZI
Course title in language of instruction: -- item not defined --
Course title in Czech: Animal Molecular Genetics
Course title in English: Animal Molecular Genetics
Mode of completion and number of credits: Exam (5 credits)
(1 ECTS credit = 28 hours of workload)
Mode of delivery/Timetabled classes: full-time, 2/2 (hours of lectures per week / hours of seminars per week)
Language of instruction: Czech
Level of course: master; master continuing
Semester: WS 2017/2018
Name of lecturer: prof. RNDr. Aleš Knoll, Ph.D. (examiner, instructor, lecturer, supervisor)
Ing. Tamara Mifková (instructor)
prof. Ing. Tomáš Urban, Ph.D. (lecturer)
Prerequisites: Genetics Z or Applied Genetics in Food Industry or Bachelor State Examination
Aims of the course:
The aim is to teach students the methodology of molecular genetics, the basic approaches of genomics, proteomics and bioinformatics, as well as to familiarize them with the results of the mapping of quantitative loci (QTL) and to explain the procedures to detect DNA polymorphism and to identify markers associated with economically important traits, especially in animal husbandry and breeding. Students master basic practical laboratory molecular genetic techniques. Next goal is to familiarize the students with practical use of genetic markers in animal selection and hybridization, including the latest techniques such as genomic selection.
Course contents:
1.Molecular genetics, DNA, pract. DNA isolation (kit) (allowance 2/2)
2.Structure of gene and genome in animals, pract. DNA sequence analysis, design of PCR primers (allowance 2/2)
3.DNA variability, genetic polymorphism, genetic markers, SNP; pract. electrophoresis - isolation control (allowance 2/2)
4.Techniques for DNA polymorphism detection (1), pract. genotype detection (RYR1) - PCR (allowance 2/2)
5.Techniques for DNA polymorphism detection (2), pract. genotype detection - elfo control of PCR products (allowance 2/2)
6.Application of molecular genetics in animal breeding (genome analysis and manipulation), the use of markers, candidate genes, pract. genotype detection - RFLP (allowance 2/2)
7.Mapping of genes and quantitative traits loci (QTL), pract. genotype detection - evaluation (allowance 2/2)
8.Genomic selection (allowance 2/2)
9.Parentity testing and individual identification in animals, pract. fragment analysis (microsatellites), problems in paternity determination, index calculations (allowance 2/2)
10.Animal cytogenetics, chromosomal aberration, chromosome staining techniques, FISH. (allowance 2/2)
a.+Excursion to the department of Animal Cytogenetics

11.Structural genomics (genome sequencing) and functional genomics; proteomics in animals, pract. DNA sequencing (allowance 2/2)
12.Current topic - animal cloning, recombinant DNA, gene and protein engineering, transgenic animals, GMO, discussion. pract. Genetic databases on the WEB and software, databases (allowance 2/2)
13.Excursion to the Institute of Animal Physiology and Genetics AS CR, Laboratory of Animal Genomics, lectures and labs (allowance 2/6)
Learning outcomes and competences:
Generic competences:
-ability to apply knowledge
-ability to solve problems
-ability to work independently
-science and research skills
-skilled at utilizing and processing information

Specific competences:
-Capability to applyknowledgeofmoleculargenetics in animal breeding
-Knowledge of genetic markers usable in the selection
-Knowledgeof basic techniquesofmoleculargenetics
-Skillsto detectbasic typesof molecular geneticmarkersusing laboratorymethods

Type of course unit: required
Year of study: Not applicable - the subject could be chosen at anytime during the course of the programme.
Work placement: There is no compulsory work placement in the course unit.
Recommended study modules: -
Learning activities and study load (hours of study load):
Type of teaching methodDaily attendance
Direct teaching
     lecture26 h
     practice6 h
     laboratory work18 h
     professional educational excursion6 h
     preparation for exam40 h
     preparation for regular assessment24 h
     report from excursion, tour, practical placement4 h
     elaboration of reports16 h
Total140 h
Assessment methods:
Exam. The exam is oral and takes 30 minutes. Student answers the two questions be drawn from a total of 30 questions. Theoretical knowledge in accordance with the syllabus is required. The knowledge of laboratory practices, including evaluation of results is tested during the exam. It is necessary to submit the protocols of laboratory exercises.
Recommended reading:
TypeAuthorTitlePublished inPublisherYearISBN
RQSNUSTAD, D P. -- SIMMONS, M J. -- MATALOVÁ, A. -- RELICHOVÁ, J. -- MENDEL, J G. et al.GenetikaBrnoMasarykova univerzita2009978-80-210-4852-2
REKNOLL, A. -- VYKOUKALOVÁ, Z.Molekulární genetika zvířat: Metody detekce polymorfizmů DNA genůBrnoMZLU200280-7157-616-6
RESIMMONS, M J. -- SNUSTAD, D P.Principles of Genetics, 5th editionNew YorkJohn Wiley & Sons2008


Last modification made by Ing. Michal Karhánek on 06/26/2017.

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