Course syllabus MGZV - Methods of Animal Genomics (FA - WS 2017/2018)


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Course code: MGZV
Course title in language of instruction: Metody genomiky zvířat
Course title in Czech: Methods of Animal Genomics
Course title in English: Methods of Animal Genomics
Mode of completion and number of credits: Exam (4 credits)
(1 ECTS credit = 28 hours of workload)
Mode of delivery/Timetabled classes: full-time, 2/1 (hours of lectures per week / hours of seminars per week)
Language of instruction: Czech
Level of course: master; master continuing
Semester: WS 2017/2018
Name of lecturer: prof. RNDr. Aleš Knoll, Ph.D. (instructor, lecturer, supervisor)
Ing. Anna Kubešová (instructor)
Prerequisites: Bachelor State Examination
 
Aims of the course:
To provide information on the use of genomic technologies and methods in the study of genomes and the use of molecular genetics in animal breeding and in research of human health. Presentation of the latest knowledge and techniques in research of genes and genomes.
 
Course contents:
1.The importance of genome study in human and animals (allowance 2/0)
2.Basic techniques in molecular biology (allowance 2/1)
3.Manipulation of nucleic acid (allowance 2/1)
4.Production of recombinant DNA (cloning), construction of gene libraries (allowance 4/6)
5.DNA amplification: PCR, AS-PCR, real-time PCR (allowance 4/2)
6.Methods for detection of DNA polymorphisms and molecular genetic markers (allowance 2/0)
7.Structural genomics and basic approaches in genome analysis (allowance 2/0)
8.Determination of parentage and identification of individuals (DNA fingerprinting)
Molecular diagnostics (of gene variants, genetic disorders), Pathogen detection (identification, typization)
(allowance 2/1)
9.Functional genomics - study of expression of genetic information (allowance 3/1)
10.Bioinformatics - genome databases and software (allowance 1/0)
 
Learning outcomes and competences:
Generic competences:
 
-ability to analyse and synthesize
-ability to apply knowledge
-ability to create new ideas (creativity)
-ability to work independently
-professional knowledge
-quality concept awareness
-science and research skills
-skilled at utilizing and processing information

Specific competences:
 
-Ability to apply knowledge of molecular genetics in animal breeding
-Ability to apply molecular genetic and genomic methods and approaches
-Knowledge of basic and advanced molecular biology methods
-Knowledge of methods for structural and functional genomics in animals
-Knowledge of modern biotechnology in animals
-Knowledge of recombinant DNA technology
-Practical skills in laboratory of molecular genetics
-Skill of identification of molecular-genetic markers

Type of course unit: required
Year of study: Not applicable - the subject could be chosen at anytime during the course of the programme.
Work placement: There is no compulsory work placement in the course unit.
Recommended study modules: -
 
Learning activities and study load (hours of study load):
Type of teaching methodDaily attendance
Direct teaching
     lecture24 h
     laboratory work12 h
Self-study
     preparation for exam56 h
     elaboration of reports20 h
Total112 h
 
Assessment methods:
Exam.
The exam consists of a written and an oral part. The written part consists of 23 questions. The written examination is reserved for 50 minutes. Each question is evaluated by 2 points. At least 30 points are needed to progress to the oral part of the exam. At the oral part, the student responds to the two questions. Theoretical knowledge according to the syllabus and the knowledge of laboratory exercises, including an explanation of the results, are required. It is necessary to submit the protocols of laboratory exercises.
 
Recommended reading:
TypeAuthorTitlePublished inPublisherYearISBN
RQŠMARDA, J. et al.Metody molekulární biologieBrnoMasarykova univerzita200580-210-3841-1
RQSENSEN, C W.Handbook of genome research: genomics, proteomics, metabolomics, bioinformatics, ethical andlegal issuesWeinheimWiley-VCH20053-527-31348-61
RERUVINSKY, A.Mammalian genomicsWallingford, Oxfordshire, UK ;CABI 0-85199-910-7
RETAGU, D. -- MOUSSARD, C.Techniques for molecular biologyEnfield, NHScience Publishers20069781578083619

RQrequired
RErecommended


Last modification made by Ing. Michal Karhánek on 06/26/2017.

Type of output: